› 8c4t

Electron Microscopy
3.23Å resolution

Hantaan virus polymerase bound to its 5' viral RNA

Released:
DOI: 10.2210/pdb8c4t/pdb
PDB entry 8c4t coloured by chain, front view.
PDB entry 8c4t coloured by chain, side view.
PDB entry 8c4t coloured by chain, top view.
Source organism: Hantaan virus 76-118
Primary publication:
Structures of active Hantaan virus polymerase uncover the mechanisms of Hantaviridae genome replication.
Durieux Trouilleton Q, Barata-García S, Arragain B, Reguera J, Malet H
Nat Commun 14 2954 (2023)
PMID: 37221161
Related structures: EMD-16428

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-217296 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
RNA-directed RNA polymerase L Chain: A
Molecule details ›
Chain: A
Length: 2173 amino acids
Theoretical weight: 249.43 KDa
Source organism: Hantaan virus 76-118
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P23456 (Residues: 1-2151; Coverage: 100%)
Gene name: L
Sequence domains:
RNA (5'-R(P*UP*AP*GP*UP*AP*GP*UP*AP*GP*AP*CP*A)-3') Chain: C
Molecule details ›
Chain: C
Length: 20 nucleotides
Theoretical weight: 6.45 KDa
Source organism: Hantaan virus 76-118
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.23Å
Relevant EMDB volumes: EMD-16428
Expression systems:
  • Trichoplusia ni
  • Not provided

Quick links

Citations

1 citations in other articles

Advances in Structural Virology via Cryo-EM in 2022.
Schoehn et al. (2023)