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› 8bps

X-ray diffraction
2.03Å resolution

Aspartate transcarbamoylase mutant (N2045C, R2238C) from Chaetomium thermophilum CAD-like in apo form

Released:
DOI: 10.2210/pdb8bps/pdb
PDB entry 8bps coloured by chain, front view.
PDB entry 8bps coloured by chain, side view.
PDB entry 8bps coloured by chain, top view.
Source organism: Chaetomium thermophilum
Primary publication:
A Tailored Strategy to Crosslink the Aspartate Transcarbamoylase Domain of the Multienzymatic Protein CAD.
Del Caño-Ochoa F, Rubio-Del-Campo A, Ramón-Maiques S
OpenAccess logo Molecules 28 (2023)
PMID: 36677714

Function and Biology Details

Reactions catalysed: 
[carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate +H(+).]
[L-glutamine + H2O = L-glutamate + NH4(+).]
[hydrogencarbonate + NH4(+) + 2 ATP = carbamoyl phosphate + 2 ADP +phosphate + 2 H(+).]
[hydrogencarbonate + L-glutamine + 2 ATP + H2O = carbamoyl phosphate +L-glutamate + 2 ADP + phosphate + 2 H(+).]
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124002 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyrimidine-specific carbamoyl phosphate synthase-aspartate carbamoyl transferase Chain: A
Molecule details ›
Chain: A
Length: 315 amino acids
Theoretical weight: 35.5 KDa
Source organism: Chaetomium thermophilum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G0S583 (Residues: 1939-2253; Coverage: 14%)
Gene name: CTHT_0032600
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 6 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P4332
Unit cell:
a: 138.829Å b: 138.829Å c: 138.829Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.166 0.205
Expression system: Escherichia coli BL21(DE3)

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