› 8bmp

Electron Microscopy
3.2Å resolution

Cryo-EM structure of the folate-specific ECF transporter complex in MSP2N2 lipid nanodiscs bound to ATP and ADP

Released:
DOI: 10.2210/pdb8bmp/pdb
PDB entry 8bmp coloured by chain, front view.
PDB entry 8bmp coloured by chain, side view.
PDB entry 8bmp coloured by chain, top view.
Source organism: Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 = JCM 1002
Primary publication:
Expulsion mechanism of the substrate-translocating subunit in ECF transporters.
Thangaratnarajah C, Nijland M, Borges-Araújo L, Jeucken A, Rheinberger J, Marrink SJ, Souza PCT, Paulino C, Slotboom DJ
Nat Commun 14 4484 (2023)
PMID: 37491368
Related structures: EMD-16120

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-172828 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Energy-coupling factor transporter ATP-binding protein EcfA1 Chain: A
Molecule details ›
Chain: A
Length: 300 amino acids
Theoretical weight: 33.17 KDa
Source organism: Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 = JCM 1002
Expression system: Escherichia coli MC1061
UniProt:
  • Canonical: Q1GBJ0 (Residues: 2-282; Coverage: 100%)
Gene names: Ldb0424, cbiO1, ecfA1
Sequence domains: ABC transporter
Energy-coupling factor transporter ATP-binding protein EcfA2 Chain: B
Molecule details ›
Chain: B
Length: 287 amino acids
Theoretical weight: 31.67 KDa
Source organism: Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 = JCM 1002
Expression system: Escherichia coli MC1061
UniProt:
  • Canonical: Q1GBI9 (Residues: 1-287; Coverage: 100%)
Gene names: Ldb0425, cbiO2, ecfA2
Sequence domains: ABC transporter
Conserved hypothetical membrane protein Chain: C
Molecule details ›
Chain: C
Length: 184 amino acids
Theoretical weight: 20.48 KDa
Source organism: Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 = JCM 1002
Expression system: Escherichia coli MC1061
UniProt:
  • Canonical: Q1G929 (Residues: 1-176; Coverage: 100%)
Gene name: Ldb1625
Sequence domains: ECF transporter, substrate-specific component
Energy-coupling factor transporter transmembrane protein EcfT Chain: D
Molecule details ›
Chain: D
Length: 265 amino acids
Theoretical weight: 30.29 KDa
Source organism: Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 = JCM 1002
Expression system: Escherichia coli MC1061
UniProt:
  • Canonical: Q1GBI8 (Residues: 1-265; Coverage: 100%)
Gene names: Ldb0426, cbiQ, ecfT
Sequence domains: Cobalt transport protein

Ligands and Environments

3 bound ligands:
Ligand ATP 1 x ATP
Ligand ADP 1 x ADP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-16120
Expression system: Escherichia coli MC1061

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