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Early transcription elongation state of influenza B/Mem polymerase backtracked due to double incoproation of nucleotide analogue T1106 and with singly incoporated T1106 at the C +1 position

Electron Microscopy
2.34Å resolution
PDB entry 8be0 coloured by chain, front view.
PDB entry 8be0 coloured by chain, side view.
PDB entry 8be0 coloured by chain, top view.
Source organism: Influenza B virus (B/Memphis/13/2003)
Assembly composition:
hetero hexamer (preferred)
Entry contents: 3 distinct polypeptide molecules
3 distinct RNA molecules

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Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero hexamer
Accessible surface area: 87580.4 Å2
Buried surface area: 47517.83 Å2
Dissociation area: 1,834.68 Å2
Dissociation energy (ΔGdiss): 22.41 kcal/mol
Dissociation entropy (TΔSdiss): 10.49 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-178420

Macromolecules

Polymerase acidic protein
Chain: A
Length: 751 amino acids
Theoretical weight: 85.82 KDa
Source organism: Influenza B virus (B/Memphis/13/2003)
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q5V8Z9 (Residues: 1-726; Coverage: 100%)
Gene name: PA
Pfam: Influenza RNA-dependent RNA polymerase subunit PA
InterPro:
Polymerase acidic protein in PDB entry 8be0, assembly 1, front view.
Polymerase acidic protein in PDB entry 8be0, assembly 1, side view.
Polymerase acidic protein in PDB entry 8be0, assembly 1, top view.
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RNA-directed RNA polymerase catalytic subunit
Chain: B
Length: 772 amino acids
Theoretical weight: 86.21 KDa
Source organism: Influenza B virus (B/Memphis/13/2003)
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q5V8Y6 (Residues: 1-752; Coverage: 100%)
Gene name: PB1
Pfam: Influenza RNA-dependent RNA polymerase subunit PB1
InterPro:
RNA-directed RNA polymerase catalytic subunit in PDB entry 8be0, assembly 1, front view.
RNA-directed RNA polymerase catalytic subunit in PDB entry 8be0, assembly 1, side view.
RNA-directed RNA polymerase catalytic subunit in PDB entry 8be0, assembly 1, top view.
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Polymerase basic protein 2
Chain: C
Length: 798 amino acids
Theoretical weight: 90.84 KDa
Source organism: Influenza B virus (B/Memphis/13/2003)
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q5V8X3 (Residues: 1-770; Coverage: 100%)
Gene name: PB2
Pfam:
InterPro:
Polymerase basic protein 2 in PDB entry 8be0, assembly 1, front view.
Polymerase basic protein 2 in PDB entry 8be0, assembly 1, side view.
Polymerase basic protein 2 in PDB entry 8be0, assembly 1, top view.
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RNA chain V
Name: 5' vRNA
Representative chains: V
Length: 14 nucleotides
Theoretical weight: 4.5 KDa
5' vRNA in PDB entry 8be0, assembly 1, front view.
5' vRNA in PDB entry 8be0, assembly 1, side view.
5' vRNA in PDB entry 8be0, assembly 1, top view.
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RNA chain R
Name: 3' vRNA
Representative chains: R
Length: 21 nucleotides
Theoretical weight: 6.7 KDa
3' vRNA in PDB entry 8be0, assembly 1, front view.
3' vRNA in PDB entry 8be0, assembly 1, side view.
3' vRNA in PDB entry 8be0, assembly 1, top view.
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RNA chain M
Name: Messenger RNA
Representative chains: M
Length: 20 nucleotides
Theoretical weight: 6.31 KDa
Messenger RNA in PDB entry 8be0, assembly 1, front view.
Messenger RNA in PDB entry 8be0, assembly 1, side view.
Messenger RNA in PDB entry 8be0, assembly 1, top view.
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