› 8b68

X-ray diffraction
1.6Å resolution

Crystal structure of UDP-glucose pyrophosphorylase from Thermocrispum agreste DSM 44070 in complex with UDP-glucose

Released:
DOI: 10.2210/pdb8b68/pdb
PDB entry 8b68 coloured by chain, front view.
PDB entry 8b68 coloured by chain, side view.
PDB entry 8b68 coloured by chain, top view.
Source organism: Thermocrispum agreste DSM 44070
Entry authors: Laustsen JU, Bento I

Function and Biology Details

Reaction catalysed: 
UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-237510 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UTP--glucose-1-phosphate uridylyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 299 amino acids
Theoretical weight: 32.13 KDa
Source organism: Thermocrispum agreste DSM 44070
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A2W4LV58 (Residues: 1-299; Coverage: 100%)
Gene name: DIU77_00305
Sequence domains: Nucleotidyl transferase

Ligands and Environments

5 bound ligands:
Ligand UPG 1 x UPG
Ligand EDO 20 x EDO
Ligand PEG 4 x PEG
Ligand SCN 1 x SCN
Ligand MG 1 x MG
1 modified residue:
Ligand CSX 2 x CSX

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: P21
Unit cell:
a: 48.036Å b: 78.433Å c: 90.289Å
α: 90° β: 93.904° γ: 90°
R-values:
R R work R free
0.178 0.176 0.204
Expression system: Escherichia coli

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