› 8avw

Electron Microscopy
3.62Å resolution

Cryo-EM structure of DrBphP in Pr state

Released:
DOI: 10.2210/pdb8avw/pdb
PDB entry 8avw coloured by chain, front view.
PDB entry 8avw coloured by chain, side view.
PDB entry 8avw coloured by chain, top view.
Source organism: Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539
Primary publication:
Structural mechanism of signal transduction in a phytochrome histidine kinase.
Wahlgren WY, Claesson E, Tuure I, Trillo-Muyo S, Bódizs S, Ihalainen JA, Takala H, Westenhoff S
Nat Commun 13 7673 (2022)
PMID: 36509762
Related structures: EMD-15685

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193365 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriophytochrome; Response regulatory domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 938 amino acids
Theoretical weight: 101.58 KDa
Source organism: Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9RZA4 (Residues: 1-755; Coverage: 100%)
  • Canonical: Q9RZA5 (Residues: 1-149; Coverage: 100%)
Gene names: DR_A0049, DR_A0050, bphP
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand LBV 2 x LBV
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.62Å
Relevant EMDB volumes: EMD-15685
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 mention without citation

Structural mechanism of signal transduction in a phytochrome histidine kinase.
Wahlgren et al. (2022)