› 8agm

X-ray diffraction
1.97Å resolution

Limonene epoxide low pH soak of epoxide hydrolase from metagenomic source ch65

Released:
DOI: 10.2210/pdb8agm/pdb
PDB entry 8agm coloured by chain, front view.
PDB entry 8agm coloured by chain, side view.
PDB entry 8agm coloured by chain, top view.
Source organism: metagenome
Entry authors: Isupov MN, De Rose SA, Mitchell D, Littlechild JA

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-226578 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chains: AAA, BBB, CCC, DDD
Molecule details ›
Chains: AAA, BBB, CCC, DDD
Length: 299 amino acids
Theoretical weight: 34.94 KDa
Source organism: metagenome
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A0A1U9WZ52 (Residues: 1-293; Coverage: 100%)
Sequence domains: alpha/beta hydrolase fold

Ligands and Environments

3 bound ligands:
Ligand LEO 8 x LEO
Ligand CL 8 x CL
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 95.985Å b: 47.416Å c: 142.814Å
α: 90° β: 91.66° γ: 90°
R-values:
R R work R free
0.233 0.232 0.27
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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