› 8ab3

X-ray diffraction
2.62Å resolution

Crystal Structure of the Lactate Dehydrogenase of Cyanobacterium Aponinum in complex with oxamate, NADH and FBP.

Released:
DOI: 10.2210/pdb8ab3/pdb
PDB entry 8ab3 coloured by chain, front view.
PDB entry 8ab3 coloured by chain, side view.
PDB entry 8ab3 coloured by chain, top view.
Source organism: Cyanobacterium aponinum
Primary publication:
Deciphering Evolutionary Trajectories of Lactate Dehydrogenases Provides New Insights into Allostery.
Robin AY, Brochier-Armanet C, Bertrand Q, Barette C, Girard E, Madern D
Mol Biol Evol 40 (2023)
PMID: 37797308

Function and Biology Details

Reaction catalysed: 
(S)-lactate + NAD(+) = pyruvate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125719 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lactate dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 337 amino acids
Theoretical weight: 37.04 KDa
Source organism: Cyanobacterium aponinum
Expression system: Escherichia coli
UniProt:
  • Canonical: K9Z684 (Residues: 1-331; Coverage: 100%)
Gene names: Cyan10605_1816, ldh
Sequence domains:

Ligands and Environments


Cofactor: Ligand NAI 4 x NAI
2 bound ligands:
Ligand OXM 4 x OXM
Ligand FBP 2 x FBP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21
Unit cell:
a: 70.369Å b: 111.504Å c: 101.793Å
α: 90° β: 99.78° γ: 90°
R-values:
R R work R free
0.188 0.186 0.232
Expression system: Escherichia coli

Quick links