› 8a4u

X-ray diffraction
1.9Å resolution

Crystal structure of human cathepsin L with CAA0225

Released:
DOI: 10.2210/pdb8a4u/pdb
PDB entry 8a4u coloured by chain, front view.
PDB entry 8a4u coloured by chain, side view.
PDB entry 8a4u coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Falke S, Lieske J, Guenther S, Reinke PYA, Ewert W, Loboda J, Karnicar K, Usenik A, Lindic N, Sekirnik A, Chapman HN, Hinrichs W, Turk D, Meents A

Function and Biology Details

Reaction catalysed: 
Similar to that of papain. As compared to cathepsin B, cathepsin L exhibits higher activity toward protein substrates, but has little activity on Z-Arg-Arg-NHMec, and no peptidyl-dipeptidase activity.
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139718 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cathepsin L Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 220 amino acids
Theoretical weight: 24.19 KDa
Source organism: Homo sapiens
Expression system: Komagataella phaffii GS115
UniProt:
  • Canonical: P07711 (Residues: 114-333; Coverage: 70%)
Gene names: CTSL, CTSL1
Sequence domains: Papain family cysteine protease

Ligands and Environments

6 bound ligands:
Ligand L2X 4 x L2X
Ligand NA 3 x NA
Ligand PEG 9 x PEG
Ligand PGE 1 x PGE
Ligand EDO 2 x EDO
Ligand PG4 1 x PG4
1 modified residue:
Ligand CSD 4 x CSD

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, DESY BEAMLINE P11
Spacegroup: P1
Unit cell:
a: 57Å b: 62.87Å c: 65.18Å
α: 104.044° β: 95.826° γ: 116.051°
R-values:
R R work R free
0.179 0.178 0.21
Expression system: Komagataella phaffii GS115

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