› 8a2v

X-ray diffraction
1.59Å resolution

Room temperature structure of the ground state of AtPhot2LOV2 in space group P43212

Released:
DOI: 10.2210/pdb8a2v/pdb
PDB entry 8a2v coloured by chain, front view.
PDB entry 8a2v coloured by chain, side view.
PDB entry 8a2v coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Slow protein dynamics probed by time-resolved oscillation crystallography at room temperature.
Aumonier S, Engilberge S, Caramello N, von Stetten D, Gotthard G, Leonard GA, Mueller-Dieckmann C, Royant A
IUCrJ 9 756-767 (2022)
PMID: 36381146

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161064 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phototropin-2 Chain: A
Molecule details ›
Chain: A
Length: 128 amino acids
Theoretical weight: 15.01 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P93025 (Residues: 387-492; Coverage: 12%)
Gene names: At5g58140, CAV1, K21L19.6, KIN7, NPL1, PHOT2
Sequence domains: PAS domain

Ligands and Environments


Cofactor: Ligand FMN 1 x FMN
1 bound ligand:
Ligand MES 1 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-3
Spacegroup: P43212
Unit cell:
a: 40.829Å b: 40.829Å c: 132.814Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.165 0.2
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Combining on-line spectroscopy with synchrotron and X-ray free electron laser crystallography.
Makita et al. (2023)
2 other articles

1 mention without citation

Slow protein dynamics probed by time-resolved oscillation crystallography at room temperature.
Aumonier et al. (2022)