› 8e9x

Electron Microscopy
2.7Å resolution

CryoEM structure of miniGo-coupled hM4Di in complex with DCZ

Released:
DOI: 10.2210/pdb8e9x/pdb
PDB entry 8e9x coloured by chain, front view.
PDB entry 8e9x coloured by chain, side view.
PDB entry 8e9x coloured by chain, top view.
Source organisms:
Primary publication:
Molecular basis for selective activation of DREADD-based chemogenetics.
Zhang S, Gumpper RH, Huang XP, Liu Y, Krumm BE, Cao C, Fay JF, Roth BL
Nature (2022)
PMID: 36450989
Related structures: EMD-27967

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-225093 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Muscarinic acetylcholine receptor M4 Chain: A
Molecule details ›
Chain: A
Length: 348 amino acids
Theoretical weight: 39.23 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P08173 (Residues: 1-479; Coverage: 73%)
Gene name: CHRM4
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Guanine nucleotide-binding protein G(o) subunit alpha Chain: B
Molecule details ›
Chain: B
Length: 225 amino acids
Theoretical weight: 25.16 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P09471 (Residues: 4-354; Coverage: 64%)
Gene name: GNAO1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: C
Molecule details ›
Chain: C
Length: 339 amino acids
Theoretical weight: 37.29 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: D
Molecule details ›
Chain: D
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
scFv16 Chain: E
Molecule details ›
Chain: E
Length: 251 amino acids
Theoretical weight: 26.68 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda

Ligands and Environments

1 bound ligand:
Ligand WEC 1 x WEC
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.7Å
Relevant EMDB volumes: EMD-27967
Expression system: Spodoptera frugiperda

Quick links

Citations

7 review citations

Psychedelics: preclinical insights provide directions for future research.
Gumpper et al. (2024)
6 more