8da6

X-ray diffraction
1.5Å resolution

Coevolved affibody-Z domain pair LL1.c5

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-235736 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Immunoglobulin G-binding protein A Chain: A
Molecule details ›
Chain: A
Length: 67 amino acids
Theoretical weight: 7.84 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P02976 (Residues: 213-269; Coverage: 12%)
Gene names: SAOUHSC_00069, spa
Sequence domains: B domain
Affibody LL1.FIIM Chains: B, D
Molecule details ›
Chains: B, D
Length: 67 amino acids
Theoretical weight: 7.7 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Immunoglobulin G-binding protein A Chain: C
Molecule details ›
Chain: C
Length: 67 amino acids
Theoretical weight: 7.81 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P02976 (Residues: 213-269; Coverage: 12%)
Gene names: SAOUHSC_00069, spa
Sequence domains: B domain

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-1
Spacegroup: P212121
Unit cell:
a: 37.016Å b: 42.105Å c: 140.805Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.216 0.251
Expression system: Escherichia coli