› 8abp

X-ray diffraction
1.49Å resolution

SUGAR-BINDING AND CRYSTALLOGRAPHIC STUDIES OF AN ARABINOSE-BINDING PROTEIN MUTANT (MET108LEU) WHICH EXHIBITS ENHANCED AFFINITY AND ALTERED SPECIFICITY

Released:
DOI: 10.2210/pdb8abp/pdb
PDB entry 8abp coloured by chain, front view.
PDB entry 8abp coloured by chain, side view.
PDB entry 8abp coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Sugar-binding and crystallographic studies of an arabinose-binding protein mutant (Met108Leu) that exhibits enhanced affinity and altered specificity.
Vermersch PS, Lemon DD, Tesmer JJ, Quiocho FA
Biochemistry 30 6861-6 (1991)
PMID: 2069949

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136385 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-arabinose-binding periplasmic protein Chain: A
Molecule details ›
Chain: A
Length: 306 amino acids
Theoretical weight: 33.23 KDa
Source organism: Escherichia coli
Expression system: Not provided
UniProt:
  • Canonical: P02924 (Residues: 24-329; Coverage: 100%)
Gene names: JW1889, araF, b1901
Sequence domains: Periplasmic binding proteins and sugar binding domain of LacI family
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand GLA 1 x GLA
Ligand GAL 1 x GAL
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 55.62Å b: 71.93Å c: 78.09Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 not available not available
Expression system: Not provided

Quick links

Citations

5 review citations

The structure and function of glutamate receptor ion channels.
Madden DR. (2002)
4 more

14 mentions without citation

Chemical shift prediction for protein structure calculation and quality assessment using an optimally parameterized force field.
Nielsen et al. (2012)
13 more