X-ray diffraction
4.2Å resolution

Pfs48/45 bound to Fab fragment of monoclonal antibody 10D8

Entry authors: Ko KT, Lennartz FL, Higgins MK

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (6 distinct):
Gametocyte surface protein P45/48 Chains: A, D
Molecule details ›
Chains: A, D
Length: 428 amino acids
Theoretical weight: 49.18 KDa
Source organism: Plasmodium falciparum
Expression system: Drosophila melanogaster
  • Canonical: Q8I6T1 (Residues: 1-428; Coverage: 95%)
Gene names: PF13_0247, PF3D7_1346700, PF45/48, PFS45-48, PFS45/48
Sequence domains: Sexual stage antigen s48/45 domain
10D8 heavy chain Chains: B, E
Molecule details ›
Chains: B, E
Length: 466 amino acids
Theoretical weight: 51.49 KDa
Source organism: Mus musculus
Expression system: Mus musculus
10D8 light chain Chains: C, F
Molecule details ›
Chains: C, F
Length: 240 amino acids
Theoretical weight: 26.54 KDa
Source organism: Mus musculus
Expression system: Mus musculus

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer : NEW Components: NAG, BMA
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Unit cell:
a: 81.771Å b: 125.803Å c: 216.601Å
α: 90° β: 90° γ: 90°
R R work R free
0.261 0.259 0.276
Expression systems:
  • Drosophila melanogaster
  • Mus musculus