› 7zx2

X-ray diffraction
2.5Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-237191 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Tubulin alpha-1B chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 451 amino acids
Theoretical weight: 50.2 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P81947 (Residues: 1-451; Coverage: 100%)
Sequence domains:
Tubulin beta-2B chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 445 amino acids
Theoretical weight: 50 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q6B856 (Residues: 1-445; Coverage: 100%)
Gene name: TUBB2B
Sequence domains:
Stathmin-4 Chain: E
Molecule details ›
Chain: E
Length: 189 amino acids
Theoretical weight: 22.13 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P63043 (Residues: 1-189; Coverage: 100%)
Gene name: Stmn4
Sequence domains: Stathmin family
Tubulin beta-2B chain Chain: F
Molecule details ›
Chain: F
Length: 384 amino acids
Theoretical weight: 44.38 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli

Ligands and Environments

10 bound ligands:
Ligand GTP 2 x GTP
Ligand MG 4 x MG
Ligand GOL 8 x GOL
Ligand CL 1 x CL
Ligand CA 3 x CA
Ligand GDP 2 x GDP
Ligand MES 1 x MES
Ligand K9I 1 x K9I
Ligand DMS 1 x DMS
Ligand ACP 1 x ACP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P212121
Unit cell:
a: 104.709Å b: 156.973Å c: 181.043Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.197 0.235
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Alternative Approaches to Understand Microtubule Cap Morphology and Function.
Oliva et al. (2023)
1 other article