› 7zoa

Electron Microscopy
4Å resolution

cryo-EM structure of CGT ABC transporter in presence of CBG substrate

Released:
DOI: 10.2210/pdb7zoa/pdb
PDB entry 7zoa coloured by chain, front view.
PDB entry 7zoa coloured by chain, side view.
PDB entry 7zoa coloured by chain, top view.
Source organism: Brucella abortus 2308
Primary publication:
Mechanism of cyclic β-glucan export by ABC transporter Cgt of Brucella.
Sedzicki J, Ni D, Lehmann F, Wu N, Zenobi R, Jung S, Stahlberg H, Dehio C
Nat Struct Mol Biol 29 1170-1177 (2022)
PMID: 36456825
Related structures: EMD-14845

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + beta-glucan(Side 1) = ADP + phosphate + beta-glucan(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174183 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Beta-(1-->2)glucan export ATP-binding/permease protein NdvA Chains: A, B
Molecule details ›
Chains: A, B
Length: 599 amino acids
Theoretical weight: 66.03 KDa
Source organism: Brucella abortus 2308
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2YQ73 (Residues: 1-599; Coverage: 100%)
Gene names: BAB1_1017, cgt, ndvA
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4Å
Relevant EMDB volumes: EMD-14845
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Immunomodulatory Effect and Biological Significance of β-Glucans.
Zhong et al. (2023)
1 more