› 7zmc

X-ray diffraction
3.1Å resolution

Ketosynthase domain of module 4 from Brevibacillus Brevis orphan BGC11

Released:
DOI: 10.2210/pdb7zmc/pdb
PDB entry 7zmc coloured by chain, front view.
PDB entry 7zmc coloured by chain, side view.
PDB entry 7zmc coloured by chain, top view.
Source organism: Brevibacillus brevis NBRC 100599
Primary publication:
The structure of a polyketide synthase bimodule core.
Tittes YU, Herbst DA, Martin SFX, Munoz-Hernandez H, Jakob RP, Maier T
Sci Adv 8 eabo6918 (2022)
PMID: 36129979

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111070 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative polyketide synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 635 amino acids
Theoretical weight: 70.07 KDa
Source organism: Brevibacillus brevis NBRC 100599
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C0ZGQ6 (Residues: 1587-2219; Coverage: 16%)
Gene name: BBR47_39880
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2221
Unit cell:
a: 73.56Å b: 191.79Å c: 293.81Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.264 0.263 0.288
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Application of artificial scaffold systems in microbial metabolic engineering.
Liu et al. (2023)
1 other article

1 mention without citation

The structure of a polyketide synthase bimodule core.
Tittes et al. (2022)