7zh9

X-ray diffraction
1.72Å resolution

Uba1 in complex with ATP

Released:

Function and Biology Details

Reaction catalysed:
ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine = AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin-activating enzyme E1 1 Chain: A
Molecule details ›
Chain: A
Length: 1024 amino acids
Theoretical weight: 114.39 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P22515 (Residues: 1-1024; Coverage: 100%)
Gene names: UBA1, YKL210W
Sequence domains:

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: C2221
Unit cell:
a: 107.72Å b: 118.064Å c: 196.654Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.165 0.206
Expression system: Escherichia coli