› 7zgo

Electron Microscopy
2.55Å resolution

Cryo-EM structure of human NKCC1 (TM domain)

Released:
DOI: 10.2210/pdb7zgo/pdb
PDB entry 7zgo coloured by chain, front view.
PDB entry 7zgo coloured by chain, side view.
PDB entry 7zgo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cryo-EM structure of the human NKCC1 transporter reveals mechanisms of ion coupling and specificity.
Neumann C, Rosenbaek LL, Flygaard RK, Habeck M, Karlsen JL, Wang Y, Lindorff-Larsen K, Gad HH, Hartmann R, Lyons JA, Fenton RA, Nissen P
EMBO J 41 e110169 (2022)
PMID: 36239040
Related structures: EMD-14709

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157148 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Solute carrier family 12 member 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 1216 amino acids
Theoretical weight: 131.82 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P55011 (Residues: 2-1212; Coverage: 100%)
Gene names: NKCC1, SLC12A2
Sequence domains:

Ligands and Environments

5 bound ligands:
Ligand NA 2 x NA
Ligand K 2 x K
Ligand CL 4 x CL
Ligand POV 7 x POV
Ligand Y01 6 x Y01
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.55Å
Relevant EMDB volumes: EMD-14709
Expression system: Homo sapiens

Quick links

Citations

4 review citations

Structural Pharmacology of Cation-Chloride Cotransporters.
Zhao et al. (2022)
3 more