› 7zg7

Electron Microscopy
1.77Å resolution

Structure of human Apoferritin obtained from ssDNA coated grid

Released:
DOI: 10.2210/pdb7zg7/pdb
PDB entry 7zg7 coloured by chain, front view.
PDB entry 7zg7 coloured by chain, side view.
PDB entry 7zg7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Polyelectrolyte coating of cryo-EM grids improves lateral distribution and prevents aggregation of macromolecules.
Hrebík D, Gondová M, Valentová L, Füzik T, Přidal A, Nováček J, Plevka P
Acta Crystallogr D Struct Biol 78 1337-1346 (2022)
PMID: 36322417
Related structures: EMD-14705

Function and Biology Details

Reaction catalysed: 
4 Fe(2+) + 4 H(+) + O(2) = 4 Fe(3+) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136244 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferritin heavy chain Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X
Length: 172 amino acids
Theoretical weight: 20.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P02794 (Residues: 6-177; Coverage: 94%)
Gene names: FTH, FTH1, FTHL6, OK/SW-cl.84, PIG15
Sequence domains: Ferritin-like domain

Ligands and Environments

2 bound ligands:
Ligand ZN 270 x ZN
Ligand NA 112 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 1.77Å
Relevant EMDB volumes: EMD-14705
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 mention without citation

Polyelectrolyte coating of cryo-EM grids improves lateral distribution and prevents aggregation of macromolecules.
Hrebík et al. (2022)