› 7zas

X-ray diffraction
2Å resolution

Crystal structure of cleaved Iripin-4 serpin from tick Ixodes ricinus

Released:
DOI: 10.2210/pdb7zas/pdb
PDB entry 7zas coloured by chain, front view.
PDB entry 7zas coloured by chain, side view.
PDB entry 7zas coloured by chain, top view.
Source organism: Ixodes ricinus
Primary publication:
Conformational transition of the Ixodes ricinus salivary serpin Iripin-4.
Kascakova B, Kotal J, Havlickova P, Vopatkova V, Prudnikova T, Grinkevich P, Kuty M, Chmelar J, Kuta Smatanova I
Acta Crystallogr D Struct Biol (2023)
PMID: 37092969

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-215551 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Iripin-4 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 341 amino acids
Theoretical weight: 37.86 KDa
Source organism: Ixodes ricinus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0K8RJV9 (Residues: 17-357; Coverage: 91%)
Sequence domains: Serpin (serine protease inhibitor)
Iripin-4 Chains: aa, bb, cc, dd
Molecule details ›
Chains: aa, bb, cc, dd
Length: 35 amino acids
Theoretical weight: 4.15 KDa
Source organism: Ixodes ricinus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0K8RJV9 (Residues: 358-392; Coverage: 9%)

Ligands and Environments

1 bound ligand:
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P21
Unit cell:
a: 65.699Å b: 138.414Å c: 80.217Å
α: 90° β: 107.7° γ: 90°
R-values:
R R work R free
0.188 0.187 0.25
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 mention without citation

Conformational transition of the Ixodes ricinus salivary serpin Iripin-4.
Kascakova et al. (2023)