› 7z6z

X-ray diffraction
1.75Å resolution

Crystal structure of Angiotensin-1 converting enzyme N-domain in complex with fosinoprilat

Released:
DOI: 10.2210/pdb7z6z/pdb
PDB entry 7z6z coloured by chain, front view.
PDB entry 7z6z coloured by chain, side view.
PDB entry 7z6z coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for the inhibition of human angiotensin-1 converting enzyme by fosinoprilat.
Cozier GE, Newby EC, Schwager SLU, Isaac RE, Sturrock ED, Acharya KR
FEBS J (2022)
PMID: 35653492

Function and Biology Details

Reaction catalysed: 
Release of a C-terminal dipeptide, oligopeptide-|-Xaa-Yaa, when Xaa is not Pro, and Yaa is neither Asp nor Glu. Thus, conversion of angiotensin I to angiotensin II, with increase in vasoconstrictor activity, but no action on angiotensin II.
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146325 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (5 distinct):
Angiotensin-converting enzyme, soluble form Chains: A, B
Molecule details ›
Chains: A, B
Length: 628 amino acids
Theoretical weight: 72.49 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P12821 (Residues: 30-657; Coverage: 49%)
Gene names: ACE, DCP, DCP1
Sequence domains: Angiotensin-converting enzyme

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
11 bound ligands:
Ligand KS8 2 x KS8
Ligand PEG 8 x PEG
Ligand PGE 3 x PGE
Ligand PG4 2 x PG4
Ligand ACT 10 x ACT
Ligand EDO 12 x EDO
Ligand ZN 2 x ZN
Ligand CL 2 x CL
Ligand CA 3 x CA
Ligand NA 1 x NA
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P1
Unit cell:
a: 72.575Å b: 77.8Å c: 81.592Å
α: 88.921° β: 64.616° γ: 74.805°
R-values:
R R work R free
0.172 0.172 0.2
Expression system: Cricetulus griseus

Quick links

Citations

2 mentions without citation

Small molecule angiotensin converting enzyme inhibitors: A medicinal chemistry perspective.
Zheng et al. (2022)
1 more