› 7yok

X-ray diffraction
2.8Å resolution

Crystal Structure of Tetra mutant (D67E, A68P, L98I, A301S) tetra mutant of O-acetyl-L-serine sulfhydrylase from Haemophilus influenzae at 2.8 A

Released:
DOI: 10.2210/pdb7yok/pdb
PDB entry 7yok coloured by chain, front view.
PDB entry 7yok coloured by chain, side view.
PDB entry 7yok coloured by chain, top view.
Source organism: Haemophilus influenzae Rd KW20
Entry authors: Saini N, Kumar N, Rahisuddin R, Singh AK

Function and Biology Details

Reaction catalysed: 
O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155240 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine synthase Chain: A
Molecule details ›
Chain: A
Length: 316 amino acids
Theoretical weight: 33.71 KDa
Source organism: Haemophilus influenzae Rd KW20
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P45040 (Residues: 1-316; Coverage: 100%)
Gene names: HI_1103, cysK
Sequence domains: Pyridoxal-phosphate dependent enzyme

Ligands and Environments

No bound ligands
1 modified residue:
Ligand LLP 1 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: I41
Unit cell:
a: 113.043Å b: 113.043Å c: 44.343Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.229 0.265
Expression system: Escherichia coli BL21(DE3)

Quick links