X-ray diffraction
2.76Å resolution

The complex of nanobody Rh57 binding to GTP-bound RhoA active form

Entry authors: Zhang YR, Liu R, Ding Y

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
RhoA Chain: A
Molecule details ›
Chain: A
Length: 183 amino acids
Theoretical weight: 20.7 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
Rh57 Chain: B
Molecule details ›
Chain: B
Length: 145 amino acids
Theoretical weight: 16.21 KDa
Source organism: Camelus bactrianus
Expression system: Escherichia coli

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL02U1
Spacegroup: I4122
Unit cell:
a: 142.03Å b: 142.03Å c: 69.26Å
α: 90° β: 90° γ: 90°
R R work R free
0.223 0.22 0.27
Expression system: Escherichia coli