› 7x0r

X-ray diffraction
1.47Å resolution

Crystal structure of substrate binding protein Lbp complexed wtih guanosine from Clostridium thermocellum

Released:
DOI: 10.2210/pdb7x0r/pdb
PDB 7x0r coloured by chain and viewed from the front.
PDB 7x0r coloured by chain and viewed from the side.
PDB 7x0r coloured by chain and viewed from the top.
Source organism: Acetivibrio thermocellus
Primary publication:
Deciphering Cellodextrin and Glucose Uptake in Clostridium thermocellum.
Yan F, Dong S, Liu YJ, Yao X, Chen C, Xiao Y, Bayer EA, Shoham Y, You C, Cui Q, Feng Y
mBio 13 e0147622 (2022)
PMID: 36069444

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lbp Chains: A, B
Molecule details ›
Chains: A, B
Length: 334 amino acids
Theoretical weight: 35.41 KDa
Source organism: Acetivibrio thermocellus
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

2 bound ligands:
Ligand GMP 2 x GMP
Ligand ZN 8 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P21
Unit cell:
a: 53.83Å b: 62.09Å c: 81.3Å
α: 90° β: 101.7° γ: 90°
R-values:
R R work R free
0.157 0.156 0.174
Expression system: Escherichia coli BL21(DE3)

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