› 7wwq

X-ray diffraction
2.72Å resolution

Crystal structure of human Ufd1-Npl4 complex

Released:
DOI: 10.2210/pdb7wwq/pdb
PDB entry 7wwq coloured by chain, front view.
PDB entry 7wwq coloured by chain, side view.
PDB entry 7wwq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for the interaction between human Npl4 and Npl4-binding motif of human Ufd1.
Nguyen TQ, My Le LT, Kim DH, Ko KS, Lee HT, Kim Nguyen YT, Kim HS, Han BW, Kang W, Yang JK
Structure (2022)
PMID: 36087575

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186161 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nuclear protein localization protein 4 homolog Chain: A
Molecule details ›
Chain: A
Length: 465 amino acids
Theoretical weight: 52.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8TAT6 (Residues: 96-560; Coverage: 77%)
Gene names: KIAA1499, NPL4, NPLOC4
Sequence domains:
Ubiquitin recognition factor in ER-associated degradation protein 1 Chain: B
Molecule details ›
Chain: B
Length: 16 amino acids
Theoretical weight: 1.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q92890 (Residues: 258-273; Coverage: 5%)
Gene names: UFD1, UFD1L

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: F222
Unit cell:
a: 152.958Å b: 165.424Å c: 192.877Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.243 0.241 0.277
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

NPLOC4 is a potential target and a poor prognostic signature in lung squamous cell carcinoma.
Wang et al. (2023)