› 7wu3

Electron Microscopy
3.1Å resolution

Cryo-EM structure of the adhesion GPCR ADGRF1 in complex with miniGs

Released:
DOI: 10.2210/pdb7wu3/pdb
PDB entry 7wu3 coloured by chain, front view.
PDB entry 7wu3 coloured by chain, side view.
PDB entry 7wu3 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of tethered agonism of the adhesion GPCRs ADGRD1 and ADGRF1.
Qu X, Qiu N, Wang M, Zhang B, Du J, Zhong Z, Xu W, Chu X, Ma L, Yi C, Han S, Shui W, Zhao Q, Wu B
Nature 604 779-785 (2022)
PMID: 35418679
Related structures: EMD-32818

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-211341 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 243 amino acids
Theoretical weight: 28.39 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63092 (Residues: 204-394; Coverage: 46%)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 351 amino acids
Theoretical weight: 38.74 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: C
Molecule details ›
Chain: C
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Nanobody Nb35 Chain: N
Molecule details ›
Chain: N
Length: 162 amino acids
Theoretical weight: 17.54 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Adhesion G-protein coupled receptor F1 Chain: R
Molecule details ›
Chain: R
Length: 674 amino acids
Theoretical weight: 74.61 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q5T601 (Residues: 251-860; Coverage: 69%)
Gene names: ADGRF1, GPR110, PGR19
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand K6G 1 x K6G
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-32818
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

8 review citations

New insights into GPCR coupling and dimerisation from cryo-EM structures.
Gusach et al. (2023)
7 more

2 mentions without citation

Regulation of pulmonary surfactant by the adhesion GPCR GPR116/ADGRF5 requires a tethered agonist-mediated activation mechanism.
Bridges et al. (2022)
1 more