› 7wkd

Electron Microscopy
3.01Å resolution

TRH-TRHR G protein complex

Released:
DOI: 10.2210/pdb7wkd/pdb
PDB entry 7wkd coloured by chain, front view.
PDB entry 7wkd coloured by chain, side view.
PDB entry 7wkd coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into ligand binding and activation of the human thyrotropin-releasing hormone receptor.
Xu Y, Cai H, You C, He X, Yuan Q, Jiang H, Cheng X, Jiang Y, Xu HE
Cell Res (2022)
PMID: 35365755
Related structures: EMD-32565

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-225607 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1; Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 361 amino acids
Theoretical weight: 41.72 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P63096 (Residues: 1-19, 32-32, 61-181, 229-242, 309-309, 319-319, 347-348; Coverage: 42%)
  • Canonical: P63092 (Residues: 27-38, 40-67, 205-251, 266-346, 348-357, 359-386, 389-394; Coverage: 54%)
Gene names: GNAI1, GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 351 amino acids
Theoretical weight: 38.74 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
scFV16 Chain: E
Molecule details ›
Chain: E
Length: 247 amino acids
Theoretical weight: 26.28 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Thyrotropin-releasing hormone receptor Chain: R
Molecule details ›
Chain: R
Length: 398 amino acids
Theoretical weight: 45.12 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P34981 (Residues: 1-398; Coverage: 100%)
Gene name: TRHR
Sequence domains: 7 transmembrane receptor (rhodopsin family)
TRH Chain: C
Molecule details ›
Chain: C
Length: 3 amino acids
Theoretical weight: 363 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
2 modified residues:
Ligand PCA 1 x PCA
Ligand LPD 1 x LPD

Experiments and Validation Details

Entry percentile scores
Resolution: 3.01Å
Relevant EMDB volumes: EMD-32565
Expression systems:
  • Trichoplusia ni
  • Not provided

Quick links

Citations

2 citation in other articles

Delineating the conformational landscape and intrinsic properties of the angiotensin II type 2 receptor using a computational study.
Cong et al. (2022)
1 more

2 mentions without citation

Ligand-induced activation and G protein coupling of prostaglandin F2α receptor.
Wu et al. (2023)
1 more