X-ray diffraction
2.56Å resolution

Structure of SARS-CoV-2 spike receptor-binding domain complexed with its receptor equine ACE2


Function and Biology Details

Reaction catalysed:
Angiotensin II + H(2)O = angiotensin-(1-7) + L-phenylalanine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Angiotensin-converting enzyme Chain: A
Molecule details ›
Chain: A
Length: 602 amino acids
Theoretical weight: 69.78 KDa
Source organism: Equus caballus
Expression system: Escherichia coli
  • Canonical: F6V9L3 (Residues: 19-614; Coverage: 76%)
Gene name: ACE2
Sequence domains: Angiotensin-converting enzyme
Spike protein S1 Chain: B
Molecule details ›
Chain: B
Length: 229 amino acids
Theoretical weight: 25.95 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Homo sapiens
  • Canonical: P0DTC2 (Residues: 319-541; Coverage: 18%)
Gene names: 2, S
Sequence domains: Betacoronavirus spike glycoprotein S1, receptor binding

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL02U1
Spacegroup: I4122
Unit cell:
a: 195.57Å b: 195.57Å c: 149.77Å
α: 90° β: 90° γ: 90°
R R work R free
0.195 0.193 0.226
Expression systems:
  • Escherichia coli
  • Homo sapiens