7w2w

X-ray diffraction
1.79Å resolution

Crystal structure of TxGH116 R786K mutant from Thermoanaerobacterium xylanolyticum with glucose

Released:

Function and Biology Details

Reaction catalysed:
D-glucosyl-N-acylsphingosine + H(2)O = D-glucose + N-acylsphingosine
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucosylceramidase Chain: A
Molecule details ›
Chain: A
Length: 842 amino acids
Theoretical weight: 96.56 KDa
Source organism: Thermoanaerobacterium xylanolyticum LX-11
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: F6BL85 (Residues: 19-806; Coverage: 98%)
Gene name: Thexy_2211
Sequence domains:

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P21212
Unit cell:
a: 177.285Å b: 54.463Å c: 83.074Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.152 0.15 0.186
Expression system: Escherichia coli BL21(DE3)