› 7w29

X-ray diffraction
2.9Å resolution

Crystal Structure of SETD3-SAH in complex with betaA-Orn73 peptide

Released:
DOI: 10.2210/pdb7w29/pdb
PDB entry 7w29 coloured by chain, front view.
PDB entry 7w29 coloured by chain, side view.
PDB entry 7w29 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Histidine methyltransferase SETD3 methylates structurally diverse histidine mimics in actin.
Hintzen JCJ, Ma H, Deng H, Witecka A, Andersen SB, Drozak J, Guo H, Qian P, Li H, Mecinović J
Protein Sci 31 e4305 (2022)
PMID: 35481649

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + protein L-histidine = S-adenosyl-L-homocysteine + protein N(tau)-methyl-L-histidine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158074 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin, cytoplasmic 1, N-terminally processed Chain: P
Molecule details ›
Chain: P
Length: 16 amino acids
Theoretical weight: 1.88 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P60709 (Residues: 66-81; Coverage: 4%)
Gene name: ACTB
Actin-histidine N-methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 499 amino acids
Theoretical weight: 57.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q86TU7 (Residues: 1-498; Coverage: 84%)
Gene names: C14orf154, SETD3
Sequence domains:

Ligands and Environments


Cofactor: Ligand SAH 1 x SAH
No bound ligands
1 modified residue:
Ligand ORN 1 x ORN

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 60.467Å b: 77.668Å c: 112.054Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.237 0.279
Expression systems:
  • Not provided
  • Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Substrate selectivity and inhibition of histidine JmjC hydroxylases MINA53 and NO66.
Türkmen et al. (2023)

1 mention without citation

Histidine methyltransferase SETD3 methylates structurally diverse histidine mimics in actin.
Hintzen et al. (2022)