› 7vvj

Electron Microscopy
3.2Å resolution

PTHrP-bound human PTH1R in complex with Gs

Released:
DOI: 10.2210/pdb7vvj/pdb
PDB entry 7vvj coloured by chain, front view.
PDB entry 7vvj coloured by chain, side view.
PDB entry 7vvj coloured by chain, top view.
Source organisms:
Primary publication:
Endogenous ligand recognition and structural transition of a human PTH receptor.
Kobayashi K, Kawakami K, Kusakizako T, Miyauchi H, Tomita A, Kobayashi K, Shihoya W, Yamashita K, Nishizawa T, Kato HE, Inoue A, Nureki O
Mol Cell 82 3468-3483.e5 (2022)
PMID: 35932760
Related structures: EMD-32141

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-225468 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 380 amino acids
Theoretical weight: 44.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63092 (Residues: 5-394; Coverage: 96%)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 351 amino acids
Theoretical weight: 38.74 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P54311 (Residues: 2-340; Coverage: 100%)
Gene name: Gnb1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 68 amino acids
Theoretical weight: 7.55 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63212 (Residues: 1-67; Coverage: 94%)
Gene name: GNG2
Sequence domains: GGL domain
nanobody Nb35 Chain: N
Molecule details ›
Chain: N
Length: 137 amino acids
Theoretical weight: 15.02 KDa
Source organism: unidentified
Expression system: Escherichia coli
PTHrP[1-36] Chain: P
Molecule details ›
Chain: P
Length: 36 amino acids
Theoretical weight: 4.27 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P12272 (Residues: 37-72; Coverage: 24%)
Gene names: PTHLH, PTHRP
Sequence domains: Parathyroid hormone family
Parathyroid hormone/parathyroid hormone-related peptide receptor Chain: R
Molecule details ›
Chain: R
Length: 482 amino acids
Theoretical weight: 55.19 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q03431 (Residues: 27-501; Coverage: 84%)
Gene names: PTH1R, PTHR, PTHR1
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-32141
Expression systems:
  • Escherichia coli
  • Spodoptera frugiperda
  • Not provided
  • Homo sapiens

Quick links

Citations

1 review citation

Parathyroid Hormone-Related Protein/Parathyroid Hormone Receptor 1 Signaling in Cancer and Metastasis.
Zhao et al. (2023)
9 other articles

2 mentions without citation

Altered signaling at the PTH receptor via modified agonist contacts with the extracellular domain provides a path to prolonged agonism in vivo.
Liu et al. (2022)
1 more