› 7vqo

Electron Microscopy
2.19Å resolution

Cryo-EM structure of Ams1 bound to the FW domain of Nbr1

Released:
DOI: 10.2210/pdb7vqo/pdb
PDB entry 7vqo coloured by chain, front view.
PDB entry 7vqo coloured by chain, side view.
PDB entry 7vqo coloured by chain, top view.
Source organisms:
Primary publication:
Structural mechanism of protein recognition by the FW domain of autophagy receptor Nbr1
Zhang J, Wang YY, Pan ZQ, Li Y, Sui J, Du LL, Ye K
Nat Commun 13 3650- (2022)
Related structures: EMD-32091

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124084 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Maltose/maltodextrin-binding periplasmic protein; ZZ-type domain-containing protein; alpha-mannosidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 1616 amino acids
Theoretical weight: 181.68 KDa
Source organisms: Expression system: Schizosaccharomyces pombe 972h-
UniProt:
  • Canonical: G0SGP6 (Residues: 1-1079; Coverage: 100%)
  • Canonical: G0SE85 (Residues: 606-746; Coverage: 17%)
  • Canonical: P0AEX9 (Residues: 27-392; Coverage: 99%)
Gene names: CTHT_0062850, CTHT_0067100, JW3994, b4034, malE
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.19Å
Relevant EMDB volumes: EMD-32091
Expression system: Schizosaccharomyces pombe 972h-

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