› 7vq9

X-ray diffraction
1.89Å resolution

Structure of MA1831 from Methanosarcina acetivorans in complex with farnesyl thiopyrophosphate and isopentyl S-thiolodiphosphate

Released:
DOI: 10.2210/pdb7vq9/pdb
PDB entry 7vq9 coloured by chain, front view.
PDB entry 7vq9 coloured by chain, side view.
PDB entry 7vq9 coloured by chain, top view.
Source organism: Methanosarcina acetivorans C2A
Primary publication:
Structural insights to a bi-functional isoprenyl diphosphate synthase that can catalyze head-to-tail and head-to-middle condensation.
Zhang L, Zhang X, Min J, Liu B, Huang JW, Yang Y, Liu W, Dai L, Yang Y, Chen CC, Guo RT
Int J Biol Macromol 214 492-499 (2022)
PMID: 35764165

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186290 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Di-trans-poly-cis-decaprenylcistransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 224 amino acids
Theoretical weight: 25.82 KDa
Source organism: Methanosarcina acetivorans C2A
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8TPS4 (Residues: 1-224; Coverage: 100%)
Gene names: MA_1831, uppS
Sequence domains: Putative undecaprenyl diphosphate synthase

Ligands and Environments

4 bound ligands:
Ligand ISY 2 x ISY
Ligand FPS 2 x FPS
Ligand MG 2 x MG
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER METALJET
Spacegroup: C2
Unit cell:
a: 111.899Å b: 81.308Å c: 60.023Å
α: 90° β: 103.46° γ: 90°
R-values:
R R work R free
0.144 0.142 0.185
Expression system: Escherichia coli BL21(DE3)

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