› 7voc

X-ray diffraction
2.62Å resolution

The crystal structure of a Radical SAM Enzyme BlsE involved in the Biosynthesis of Blasticidin S

Released:
DOI: 10.2210/pdb7voc/pdb
PDB 7voc coloured by chain and viewed from the front.
PDB 7voc coloured by chain and viewed from the side.
PDB 7voc coloured by chain and viewed from the top.
Source organism: Streptomyces griseochromogenes
Primary publication:
Radical S-Adenosyl Methionine Enzyme BlsE Catalyzes a Radical-Mediated 1,2-Diol Dehydration during the Biosynthesis of Blasticidin S.
Lee YH, Hou X, Chen R, Feng J, Liu X, Ruszczycky MW, Gao JM, Wang B, Zhou J, Liu HW
J Am Chem Soc 144 4478-4486 (2022)
PMID: 35238201

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Radical SAM core domain-containing protein Chain: C
Molecule details ›
Chain: C
Length: 364 amino acids
Theoretical weight: 40.33 KDa
Source organism: Streptomyces griseochromogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1B1AYF2 (Residues: 1-344; Coverage: 100%)
Gene name: AVL59_19980
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand SF4 2 x SF4
Ligand SAM 1 x SAM
Ligand 7QO 1 x 7QO
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: I4122
Unit cell:
a: 163.92Å b: 163.92Å c: 158.81Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.214 0.234
Expression system: Escherichia coli

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