7vdt

Electron Microscopy
2.8Å resolution

The motor-nucleosome module of human chromatin remodeling PBAF-nucleosome complex

Released:
Source organisms:
Primary publication:
Structure of human chromatin-remodelling PBAF complex bound to a nucleosome.
Nature 605 166-171 (2022)
PMID: 35477757
Related structures: EMD-31925

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
PDBe Complex ID:
PDB-CPX-122222 (preferred)
Entry contents:
5 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (7 distinct):
Transcription activator BRG1 Chain: A
Molecule details ›
Chain: A
Length: 1485 amino acids
Theoretical weight: 169.6 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P51532 (Residues: 160-1647; Coverage: 88%)
Gene names: BAF190A, BRG1, SMARCA4, SNF2B, SNF2L4
Sequence domains:
Histone H4 Chains: B, F
Molecule details ›
Chains: B, F
Length: 103 amino acids
Theoretical weight: 11.39 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P62799 (Residues: 1-103; Coverage: 100%)
Sequence domains: Centromere kinetochore component CENP-T histone fold
Histone H2A type 1 Chains: C, G
Molecule details ›
Chains: C, G
Length: 130 amino acids
Theoretical weight: 14.11 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P06897 (Residues: 1-130; Coverage: 100%)
Sequence domains:
Histone H2B 1.1 Chains: D, H
Molecule details ›
Chains: D, H
Length: 126 amino acids
Theoretical weight: 13.97 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P02281 (Residues: 1-126; Coverage: 100%)
Sequence domains: Core histone H2A/H2B/H3/H4
Histone H3.2 Chains: E, K
Molecule details ›
Chains: E, K
Length: 136 amino acids
Theoretical weight: 15.44 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P84233 (Residues: 1-136; Coverage: 100%)
Sequence domains: Core histone H2A/H2B/H3/H4
DNA (207-MER) Chain: I
Molecule details ›
Chain: I
Length: 207 nucleotides
Theoretical weight: 63.68 KDa
DNA (207-MER) Chain: J
Molecule details ›
Chain: J
Length: 207 nucleotides
Theoretical weight: 64.15 KDa

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.8Å
Relevant EMDB volumes: EMD-31925
Expression systems:
  • Homo sapiens
  • Escherichia coli