› 7vcm

X-ray diffraction
1.85Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142351 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein; Potassium binding protein Kbp Chains: A, B
Molecule details ›
Chains: A, B
Length: 386 amino acids
Theoretical weight: 43.01 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 2-144, 149-238; Coverage: 98%)
  • Canonical: P0ADE6 (Residues: 2-149; Coverage: 99%)
Gene names: GFP, JW2640, b2665, kbp, ygaU, yzzM
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand K 2 x K
1 modified residue:
Ligand CRO 2 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P1
Unit cell:
a: 46.827Å b: 49.319Å c: 83.683Å
α: 89.96° β: 89.97° γ: 80.95°
R-values:
R R work R free
0.195 0.195 0.225
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Genetically encoded fluorescent sensors for metals in biology.
Torres-Ocampo et al. (2023)
2 more

1 mention without citation

A sensitive and specific genetically-encoded potassium ion biosensor for in vivo applications across the tree of life.
Wu et al. (2022)