7vc5

X-ray diffraction
1.61Å resolution

Toxoplasma gondii Prolyl-tRNA Synthetase (TgPRS) in Complex with inhibitor T35 and Febrifugine (FF) at 1.61 A resolution

Released:
Source organism: Toxoplasma gondii
Entry authors: Manickam Y, Malhotra N, Sharma A

Function and Biology Details

Reaction catalysed:
ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
proline--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 500 amino acids
Theoretical weight: 57.94 KDa
Source organism: Toxoplasma gondii
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A7J6JUK2 (Residues: 215-711; Coverage: 70%)
Gene name: TGRH88_057780
Sequence domains:

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: C2
Unit cell:
a: 115.194Å b: 73.092Å c: 74.936Å
α: 90° β: 111.57° γ: 90°
R-values:
R R work R free
0.166 0.165 0.196
Expression system: Escherichia coli