› 7vc5

X-ray diffraction
1.61Å resolution

Toxoplasma gondii Prolyl-tRNA Synthetase (TgPRS) in Complex with inhibitor T35 and Febrifugine (FF) at 1.61 A resolution

Released:
DOI: 10.2210/pdb7vc5/pdb
PDB 7vc5 coloured by chain and viewed from the front.
PDB 7vc5 coloured by chain and viewed from the side.
PDB 7vc5 coloured by chain and viewed from the top.
Source organism: Toxoplasma gondii
Entry authors: Manickam Y, Malhotra N, Sharma A

Function and Biology Details

Reaction catalysed: 
ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
proline--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 500 amino acids
Theoretical weight: 57.94 KDa
Source organism: Toxoplasma gondii
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A7J6JUK2 (Residues: 215-711; Coverage: 70%)
Gene name: TGRH88_057780
Sequence domains:

Ligands and Environments

5 bound ligands:
Ligand 1TI 1 x 1TI
Ligand 9SF 1 x 9SF
Ligand MES 1 x MES
Ligand EDO 7 x EDO
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: C2
Unit cell:
a: 115.194Å b: 73.092Å c: 74.936Å
α: 90° β: 111.57° γ: 90°
R-values:
R R work R free
0.166 0.165 0.196
Expression system: Escherichia coli

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