› 7v6a

Electron Microscopy
3.6Å resolution

Cry-EM structure of M4-c110-G protein complex

Released:
DOI: 10.2210/pdb7v6a/pdb
PDB entry 7v6a coloured by chain, front view.
PDB entry 7v6a coloured by chain, side view.
PDB entry 7v6a coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The unconventional activation of the muscarinic acetylcholine receptor M4R by diverse ligands.
Wang J, Wu M, Chen Z, Wu L, Wang T, Cao D, Wang H, Liu S, Xu Y, Li F, Liu J, Chen N, Zhao S, Cheng J, Wang S, Hua T
Nat Commun 13 2855 (2022)
PMID: 35606397
Related structures: EMD-31740

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-225100 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 356 amino acids
Theoretical weight: 40.56 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63096 (Residues: 1-354; Coverage: 100%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 339 amino acids
Theoretical weight: 37.29 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: C
Molecule details ›
Chain: C
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
scFv16 Chain: S
Molecule details ›
Chain: S
Length: 259 amino acids
Theoretical weight: 27.78 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Muscarinic acetylcholine receptor M4 Chain: R
Molecule details ›
Chain: R
Length: 346 amino acids
Theoretical weight: 39.06 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P08173 (Residues: 1-479; Coverage: 72%)
Gene name: CHRM4
Sequence domains: 7 transmembrane receptor (rhodopsin family)

Ligands and Environments

1 bound ligand:
Ligand 5XI 1 x 5XI
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-31740
Expression system: Spodoptera frugiperda

Quick links

Citations

2 review citations

A Novel "Activation Switch" Motif Common to All Aminergic Receptors.
Lee et al. (2023)
1 more

1 mention without citation

Assessment of Quercetin Antiemetic Properties: In Vivo and In Silico Investigations on Receptor Binding Affinity and Synergistic Effects.
Chowdhury et al. (2023)