7v4o

X-ray diffraction
1.65Å resolution

Unique non-heme hydroxylase in biosynthesis of nucleoside antibiotic pathway uncover mechanism of reaction

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Asp_Arg_Hydrox domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 182 amino acids
Theoretical weight: 21 KDa
Source organism: Streptomyces sp. MK730-62F2
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpGUT1
UniProt:
  • Canonical: C4NCJ7 (Residues: 1-182; Coverage: 100%)
Gene name: cpz10
Sequence domains: Aspartyl/Asparaginyl beta-hydroxylase

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE TPS 05A
Spacegroup: C2
Unit cell:
a: 150.839Å b: 39.035Å c: 76.591Å
α: 90° β: 119.34° γ: 90°
R-values:
R R work R free
0.182 0.18 0.214
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpGUT1