X-ray diffraction
2.1Å resolution

ZIKV NS3helicase in complex with ssRNA and ATP-Mn2+

Source organisms:
Entry authors: Lin MM, Yang HT

Function and Biology Details

Reactions catalysed:
S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-[mRNA]
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA]
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Serine protease NS3 Chain: A
Molecule details ›
Chain: A
Length: 440 amino acids
Theoretical weight: 49.59 KDa
Source organism: Zika virus
Expression system: Escherichia coli
  • Canonical: A0A024B7W1 (Residues: 1680-2119; Coverage: 13%)
Sequence domains: Flavivirus DEAD domain
RNA (5'-R(*AP*GP*AP*UP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 5 nucleotides
Theoretical weight: 1.57 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P21
Unit cell:
a: 52.62Å b: 72.356Å c: 59.507Å
α: 90° β: 94.154° γ: 90°
R R work R free
0.191 0.189 0.239
Expression systems:
  • Escherichia coli
  • Not provided