› 7v1q

X-ray diffraction
1.58Å resolution

Leifsonia Alcohol Dehydrogenases LnADH

Released:
DOI: 10.2210/pdb7v1q/pdb
PDB entry 7v1q coloured by chain, front view.
PDB entry 7v1q coloured by chain, side view.
PDB entry 7v1q coloured by chain, top view.
Source organism: Leifsonia antarctica
Primary publication:
Engineering Leifsonia Alcohol Dehydrogenase for Thermostability and Catalytic Efficiency by Enhancing Subunit Interactions.
Zhu L, Song Y, Chang C, Ma H, Yang L, Deng Z, Deng W, Qu X
Chembiochem 22 3178-3183 (2021)
PMID: 34549865

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197523 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative 2,5-dichloro-2,5-cyclohexadiene-1,4-diol dehydrogenase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 251 amino acids
Theoretical weight: 25.26 KDa
Source organism: Leifsonia antarctica
Expression system: Escherichia coli
UniProt:
  • Canonical: U2RWV7 (Residues: 1-251; Coverage: 100%)
Gene name: N136_00636
Sequence domains: Enoyl-(Acyl carrier protein) reductase

Ligands and Environments

3 bound ligands:
Ligand IPA 8 x IPA
Ligand NAD 8 x NAD
Ligand ZN 13 x ZN
1 modified residue:
Ligand MSE 32 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NFPSS BEAMLINE BL19U1
Spacegroup: P21
Unit cell:
a: 54.671Å b: 238.522Å c: 68.921Å
α: 90° β: 113.35° γ: 90°
R-values:
R R work R free
0.166 0.164 0.195
Expression system: Escherichia coli

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