› 7um6

Electron Microscopy
2.79Å resolution

CryoEM structure of Go-coupled 5-HT5AR in complex with Lisuride

Released:
DOI: 10.2210/pdb7um6/pdb
PDB entry 7um6 coloured by chain, front view.
PDB entry 7um6 coloured by chain, side view.
PDB entry 7um6 coloured by chain, top view.
Source organisms:
Primary publication:
Inactive and active state structures template selective tools for the human 5-HT5A receptor.
Zhang S, Chen H, Zhang C, Yang Y, Popov P, Liu J, Krumm BE, Cao C, Kim K, Xiong Y, Katritch V, Shoichet BK, Jin J, Fay JF, Roth BL
Nat Struct Mol Biol 29 677-687 (2022)
PMID: 35835867
Related structures: EMD-26598

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-225657 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
5-hydroxytryptamine receptor 5A Chain: A
Molecule details ›
Chain: A
Length: 326 amino acids
Theoretical weight: 36.88 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P47898 (Residues: 32-357; Coverage: 91%)
Gene name: HTR5A
Sequence domains: 7 transmembrane receptor (rhodopsin family)
miniGo protein Chain: B
Molecule details ›
Chain: B
Length: 225 amino acids
Theoretical weight: 25.16 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: C
Molecule details ›
Chain: C
Length: 339 amino acids
Theoretical weight: 37.29 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: D
Molecule details ›
Chain: D
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Single-chain variable fragment scFv16 Chain: E
Molecule details ›
Chain: E
Length: 251 amino acids
Theoretical weight: 26.68 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda

Ligands and Environments

1 bound ligand:
Ligand H8G 1 x H8G
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.79Å
Relevant EMDB volumes: EMD-26598
Expression system: Spodoptera frugiperda

Quick links

Citations

5 review citations

A Novel "Activation Switch" Motif Common to All Aminergic Receptors.
Lee et al. (2023)
4 more

1 mention without citation

Inactive and active state structures template selective tools for the human 5-HT5A receptor.
Zhang et al. (2022)