› 7uim

Electron Microscopy
3.1Å resolution

CryoEM Structure of an Group II Intron Retroelement (apo-complex)

Released:
DOI: 10.2210/pdb7uim/pdb
PDB entry 7uim coloured by chain, front view.
PDB entry 7uim coloured by chain, side view.
PDB entry 7uim coloured by chain, top view.
Source organism: [Eubacterium] rectale
Primary publication:
Structures of a mobile intron retroelement poised to attack its structured DNA target.
Chung K, Xu L, Chai P, Peng J, Devarkar SC, Pyle AM
Science 378 627-634 (2022)
PMID: 36356138
Related structures: EMD-26549

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103119 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Reverse transcriptase domain-containing protein Chain: D
Molecule details ›
Chain: D
Length: 427 amino acids
Theoretical weight: 49.08 KDa
Source organism: [Eubacterium] rectale
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A173ZME3 (Residues: 1-427; Coverage: 100%)
Gene names: ERS852417_00966, FYL37_05080, ltrA, ltrA_2
Sequence domains:
E.r IIC Intron Chain: B
Molecule details ›
Chain: B
Length: 638 nucleotides
Theoretical weight: 206.78 KDa
Source organism: [Eubacterium] rectale
Expression system: Not provided
Sequence domains:
  • Group II catalytic intron D1-D4-3
  • Group II catalytic intron D1-D4-1
  • Group II catalytic intron

    • Ligands and Environments

    2 bound ligands:
    Ligand MG 13 x MG
    Ligand NH4 2 x NH4
    No modified residues

    Experiments and Validation Details

    Entry percentile scores
    Resolution: 3.1Å
    Relevant EMDB volumes: EMD-26549
    Expression systems:
    • Escherichia coli
    • Not provided

    Quick links

    Citations

    3 citation in other articles

    Structure of the R2 non-LTR retrotransposon initiating target-primed reverse transcription.
    Wilkinson et al. (2023)
    2 more