› 7uhy

Electron Microscopy
3.66Å resolution

Human GATOR2 complex

Released:
DOI: 10.2210/pdb7uhy/pdb
PDB entry 7uhy coloured by chain, front view.
PDB entry 7uhy coloured by chain, side view.
PDB entry 7uhy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the nutrient-sensing hub GATOR2.
Valenstein ML, Rogala KB, Lalgudi PV, Brignole EJ, Gu X, Saxton RA, Chantranupong L, Kolibius J, Quast JP, Sabatini DM
Nature 607 610-616 (2022)
PMID: 35831510
Related structures: EMD-26519

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero icosamer (preferred)
PDBe Complex ID:
PDB-CPX-157305 (preferred)
Entry contents:
7 distinct polypeptide molecules
Macromolecules (7 distinct):
GATOR2 complex protein MIOS Chains: A, B
Molecule details ›
Chains: A, B
Length: 894 amino acids
Theoretical weight: 100.63 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9NXC5 (Residues: 1-875; Coverage: 100%)
Gene name: MIOS
Sequence domains:
GATOR2 complex protein WDR24 Chain: C
Molecule details ›
Chain: C
Length: 790 amino acids
Theoretical weight: 88.33 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q96S15 (Residues: 1-790; Coverage: 100%)
Gene names: C16orf21, JFP7, WDR24
Sequence domains: WD domain, G-beta repeat
GATOR2 complex protein WDR59 Chain: D
Molecule details ›
Chain: D
Length: 974 amino acids
Theoretical weight: 109.94 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q6PJI9 (Residues: 1-974; Coverage: 100%)
Gene names: FP977, KIAA1923, WDR59
Sequence domains:
Nucleoporin SEH1 Chains: E, F, G
Molecule details ›
Chains: E, F, G
Length: 421 amino acids
Theoretical weight: 46.64 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q96EE3 (Residues: 1-357; Coverage: 99%)
Gene names: SEC13L, SEH1, SEH1L
Sequence domains: WD domain, G-beta repeat
Protein SEC13 homolog Chain: H
Molecule details ›
Chain: H
Length: 322 amino acids
Theoretical weight: 35.58 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P55735 (Residues: 1-322; Coverage: 100%)
Gene names: D3S1231E, SEC13, SEC13A, SEC13L1, SEC13R
Sequence domains: WD domain, G-beta repeat
Unknown Chain: I
Molecule details ›
Chain: I
Length: 15 amino acids
Theoretical weight: 1.29 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Unknown Chain: J
Molecule details ›
Chain: J
Length: 8 amino acids
Theoretical weight: 699 Da
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

1 bound ligand:
Ligand ZN 16 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.66Å
Relevant EMDB volumes: EMD-26519
Expression system: Homo sapiens

Quick links

Citations

4 review citations

Longin domain GAP complexes in nutrient signalling, membrane traffic and neurodegeneration.
Jansen et al. (2023)
3 more

2 mentions without citation

An evolutionary mechanism to assimilate new nutrient sensors into the mTORC1 pathway.
Liu et al. (2023)
1 more