› 7uhw

X-ray diffraction
1.3Å resolution

Horse liver alcohol dehydrogenase G173A, complexed with NAD+ and 2,3,4,5,6-pentafluorobenzyl alcohol at 120 K

Released:
DOI: 10.2210/pdb7uhw/pdb
PDB entry 7uhw coloured by chain, front view.
PDB entry 7uhw coloured by chain, side view.
PDB entry 7uhw coloured by chain, top view.
Source organism: Equus caballus
Primary publication:
Dependence of crystallographic atomic displacement factors on temperature (25-150 K) for complexes of horse liver alcohol dehydrogenases
Plapp BV, Gakhar L, Subramanian R
Acta Crystallogr. D Biol. Crystallogr. D78 1221-1234 (2022)

Function and Biology Details

Reaction catalysed: 
A primary alcohol + NAD(+) = an aldehyde + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132405 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alcohol dehydrogenase E chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 374 amino acids
Theoretical weight: 39.87 KDa
Source organism: Equus caballus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00327 (Residues: 2-375; Coverage: 100%)
Sequence domains:

Ligands and Environments


Cofactor: Ligand NAJ 2 x NAJ
3 bound ligands:
Ligand ZN 4 x ZN
Ligand PFB 2 x PFB
Ligand MRD 4 x MRD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P1
Unit cell:
a: 44.37Å b: 51.55Å c: 92.57Å
α: 91.8° β: 103.02° γ: 110.19°
R-values:
R R work R free
0.129 0.129 0.165
Expression system: Escherichia coli

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