› 7uhv

X-ray diffraction
1.3Å resolution

Horse liver alcohol dehydrogenase G173A, complexed with NAD+ and 2,3,4,5,6-pentafluorobenzyl alcohol at 50 K

Released:
DOI: 10.2210/pdb7uhv/pdb
PDB 7uhv coloured by chain and viewed from the front.
PDB 7uhv coloured by chain and viewed from the side.
PDB 7uhv coloured by chain and viewed from the top.
Source organism: Equus caballus
Entry authors: Plapp BV, Gakhar L

Function and Biology Details

Reaction catalysed: 
A primary alcohol + NAD(+) = an aldehyde + NADH
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alcohol dehydrogenase E chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 374 amino acids
Theoretical weight: 39.87 KDa
Source organism: Equus caballus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00327 (Residues: 2-375; Coverage: 100%)
Sequence domains:

Ligands and Environments


Cofactor: Ligand NAJ 2 x NAJ
3 bound ligands:
Ligand ZN 4 x ZN
Ligand PFB 2 x PFB
Ligand MRD 4 x MRD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P1
Unit cell:
a: 44.3Å b: 51.48Å c: 92.32Å
α: 91.89° β: 103.04° γ: 110.16°
R-values:
R R work R free
0.13 0.13 0.167
Expression system: Escherichia coli

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