X-ray diffraction
1.95Å resolution

Crystal Structure of enolase family protein from Naegleria fowleri with bound 2-phosphoglyceric acid

Source organism: Naegleria fowleri
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed:
2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Phosphopyruvate hydratase Chains: A, B
Molecule details ›
Chains: A, B
Length: 477 amino acids
Theoretical weight: 52.36 KDa
Source organism: Naegleria fowleri
Expression system: Escherichia coli BL21(DE3)
  • Canonical: A0A6A5BXC3 (Residues: 17-485; Coverage: 97%)
Gene name: FDP41_001645
Sequence domains:

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P41212
Unit cell:
a: 96.86Å b: 96.86Å c: 216.64Å
α: 90° β: 90° γ: 90°
R R work R free
0.154 0.153 0.191
Expression system: Escherichia coli BL21(DE3)