› 7ugh

X-ray diffraction
1.95Å resolution

Crystal Structure of enolase family protein from Naegleria fowleri with bound 2-phosphoglyceric acid

Released:
DOI: 10.2210/pdb7ugh/pdb
PDB entry 7ugh coloured by chain, front view.
PDB entry 7ugh coloured by chain, side view.
PDB entry 7ugh coloured by chain, top view.
Source organism: Naegleria fowleri
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105528 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
phosphopyruvate hydratase Chains: A, B
Molecule details ›
Chains: A, B
Length: 477 amino acids
Theoretical weight: 52.36 KDa
Source organism: Naegleria fowleri
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A6A5BXC3 (Residues: 17-485; Coverage: 97%)
Gene name: FDP41_001645
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand 2PG 2 x 2PG
Ligand EDO 6 x EDO
Ligand ACT 4 x ACT
Ligand NA 4 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P41212
Unit cell:
a: 96.86Å b: 96.86Å c: 216.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.153 0.191
Expression system: Escherichia coli BL21(DE3)

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