› 7u6l

X-ray diffraction
2.6Å resolution

Pseudooxynicotine amine oxidase

Released:
DOI: 10.2210/pdb7u6l/pdb
PDB entry 7u6l coloured by chain, front view.
PDB entry 7u6l coloured by chain, side view.
PDB entry 7u6l coloured by chain, top view.
Source organism: Pseudomonas putida S16
Primary publication:
The enzyme pseudooxynicotine amine oxidase from Pseudomonas putida S16 is not an oxidase, but a dehydrogenase.
Choudhary V, Wu K, Zhang Z, Dulchavsky M, Barkman T, Bardwell JCA, Stull F
J Biol Chem 102251 (2022)
PMID: 35835223

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123700 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pseudooxynicotine dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 453 amino acids
Theoretical weight: 50.27 KDa
Source organism: Pseudomonas putida S16
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: F8G0P1 (Residues: 45-496; Coverage: 100%)
Gene names: PPS_4080, pnao
Sequence domains: Flavin containing amine oxidoreductase

Ligands and Environments

1 bound ligand:
Ligand FAD 4 x FAD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21
Unit cell:
a: 143.99Å b: 50.34Å c: 150.89Å
α: 90° β: 107.9° γ: 90°
R-values:
R R work R free
0.172 0.17 0.22
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

2 citation in other articles

Binding Interface and Electron Transfer Between Nicotine Oxidoreductase and Its Cytochrome c Electron Acceptor.
Mumby et al. (2022)
1 more

1 mention without citation

The enzyme pseudooxynicotine amine oxidase from Pseudomonas putida S16 is not an oxidase, but a dehydrogenase.
Choudhary et al. (2022)