› 7u4g

X-ray diffraction
1.65Å resolution

Neuraminidase from influenza virus A/Shandong/9/1993(H3N2)

Released:
DOI: 10.2210/pdb7u4g/pdb
PDB entry 7u4g coloured by chain, front view.
PDB entry 7u4g coloured by chain, side view.
PDB entry 7u4g coloured by chain, top view.
Source organism: Influenza A virus (A/Shandong/9/1993(H3N2))
Primary publication:
Prevalence and mechanisms of evolutionary contingency in human influenza H3N2 neuraminidase.
Lei R, Tan TJC, Hernandez Garcia A, Wang Y, Diefenbacher M, Teo C, Gopan G, Tavakoli Dargani Z, Teo QW, Graham CS, Brooke CB, Nair SK, Wu NC
Nat Commun 13 6443 (2022)
PMID: 36307418

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-216561 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Neuraminidase Chain: A
Molecule details ›
Chain: A
Length: 469 amino acids
Theoretical weight: 52.19 KDa
Source organism: Influenza A virus (A/Shandong/9/1993(H3N2))
Expression system: unidentified baculovirus
UniProt:
  • Canonical: B8K0N8 (Residues: 1-469; Coverage: 100%)
Gene name: NA
Sequence domains: Neuraminidase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand ZMR 1 x ZMR
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P4212
Unit cell:
a: 107.954Å b: 107.954Å c: 78.619Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.188 0.215
Expression system: unidentified baculovirus

Quick links

Citations

1 review citation

Co-evolution of immunity and seasonal influenza viruses.
Han et al. (2023)
4 other articles